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Using the program LUCIA Cytogenetics it is possible to display the entire genome in colors using the MFISH method.
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LUCIA Cytogenetics can work with fluorescent probe kits from different manufacturers.
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Get high-quality images with scientific digital CCD cameras with 14bits analog-to-digital conversion.
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The automatic camera exposure control ensures that maximum information is transferred to the computer, no detail gets lost.
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The complete control over a motorized microscope speeds up the image acquisition and reduces the risk of human-caused errors.
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Predefined acquisition settings for various probe kits.
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See any combination of the individual color images composed into one.
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See the rearrangements in pseudo color from the acquired image to the karyogram.
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Labeling scheme indicators will always help you to stay informed and understand each color stain.
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Automatic color classification including fast pseudo color display on the raw image.
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Automatic and manual spot filtering.
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Rearrangement analysis allows to quickly show only the material from the selected chromosomes and thus helps clearly reveal the rearrangement.
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Profile graphs allow to study the intensity profiles for all colors.
When applying MFISH (multiplex fluorescence in situ hybridization), images of chromosomes marked with 5 different fluorophores are captured separately using optical filters. LUCIA Cytogenetics then combines these images into one, in which each chromosome is assigned a distinct color based on the composition of the fluorophore.
MFISH complements the standard cytogenetic methods. It is very helpful in deciphering complex chromosomal rearrangements. It is used to identify non-random structural chromosome rearrangements not detectable by other methods.
MFISH allows rapid identification of simple and complex chromosomal alterations in metaphase spreads which may be associated with a disease initiation and progression. Therefore the applications of MFISH imaging are spreading fast - particularly in the field of cancer research.
Since M-FISH (24-color FISH) is based on chromosome painting it can only be applied to metaphases.
Module LUCIA Cytogenetics - MFISH is fully compatible and can be integrated with other LUCIA products: